2CMN
A Proximal Arginine Residue in the Switching Mechanism of the FixL Oxygen Sensor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-20 |
Detector | CUSTOM |
Spacegroup name | H 3 2 |
Unit cell lengths | 127.117, 127.117, 58.569 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lsw |
RMSD bond length | 0.012 |
RMSD bond angle | 1.693 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.070 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.060 | 0.740 |
Number of reflections | 8187 | |
<I/σ(I)> | 36.2 | 2.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.4 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | CRYSTALS OF THE MET-R206A BJFIXLH MUTANT WERE OBTAINED AT 4 DEGREES C VIA THE HANGING-DROP VAPOR-DIFFUSION METHOD BY MIXING EQUAL AMOUNTS OF PROTEIN (0.6-1.2 MM IN 5.0 MM TRIS-HCL, PH 8.0) AND WELL SOLUTION (50 MM HEPES, 4.5 M NACL, 5% (V/) 2-METHYL-2,4-PENTANEDIOL, PH 7.5) AND INCUBATING THE DROP OVER 0.7 ML OF WELL SOLUTION. |