2CMK
CYTIDINE MONOPHOSPHATE KINASE IN COMPLEX WITH CYTIDINE-DI-PHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 77.268, 77.268, 81.232 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.000 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NON LIGATED CMPK |
| RMSD bond length | 0.012 |
| RMSD bond angle | 23.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.84) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 * | 0.425 * |
| Total number of observations | 286435 * | |
| Number of reflections | 17252 | |
| <I/σ(I)> | 25.7 | 3 |
| Completeness [%] | 98.4 | 95.9 |
| Redundancy | 16.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 | pH 7.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | CDP | 20 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 0.4 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.5 (M) |
