2CMF
Torpedo californica acetylcholinesterase complexed with alkylene- linked bis-tacrine dimer (5 carbon linker)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.153, 113.153, 136.692 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.470 - 2.500 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.050 | 0.380 |
| Number of reflections | 34257 | |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 96.6 | 96.7 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 277 | 28-33% V/V PEG 200, 0.5MM MES PH5.8, 4DEG C, SEEDING, pH 5.80 |
