2CMD

THE CRYSTAL STRUCTURE OF E.COLI MALATE DEHYDROGENASE: A COMPLEX OF THE APOENZYME AND CITRATE AT 1.87 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	116.950, 42.900, 84.050
Unit cell angles	90.00, 130.21, 90.00

Refinement procedure

Resolution	8.000 - 1.870
R-factor	0.188
Rwork	0.188
RMSD bond length	0.013
RMSD bond angle	1.362
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	2.000 *
High resolution limit [Å]	1.750 *
Rmerge	0.059 *
Total number of observations	128427 *
Number of reflections	26366 *
Completeness [%]	99.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.6 (mg/ml)
10	1	reservoir		0.05 (%)
11	1	reservoir	PEG8000	15-18 (%)
2	1	drop	PEG8000	14-16 (%(w/v))
3	1	drop	sodium citrate	10 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop	DTT	2 (mM)
6	1	drop		0.05 (%)
7	1	reservoir	sodium citrate	10 (mM)
8	1	reservoir	EDTA	1 (mM)
9	1	reservoir	DTT	2 (mM)