2CM4
The complement inhibitor OmCI in complex with ricinoleic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-31 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.170, 55.520, 60.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.900 - 1.900 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qft |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.821 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | TNT (5.6.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.900 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 110764 | |
| <I/σ(I)> | 6.5 | 1.6 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 8.9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 30% PEG 4000 0.1 M SODIUM ACETATE PH 4.6 0.2 M AMMONIUM ACETATE |
