2CM0
The PUB domain functions as a p97 binding module in human peptide N-glycanase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Collection date | 2006-04-01 |
| Spacegroup name | P 65 |
| Unit cell lengths | 73.987, 73.987, 32.994 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.1851 |
| Rwork | 0.185 |
| R-free | 0.22310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ccq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.247 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.480 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.280 |
| Number of reflections | 8005 | |
| <I/σ(I)> | 12.8 | 4 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.2 | 50% PEG 400, 0.1M CHES, PH 9.5, 0.2M NACL |
