2CLU

Recombinant human H ferritin, K86Q and E107D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-19
Detector	MARRESEARCH
Spacegroup name	F 4 3 2
Unit cell lengths	180.970, 180.970, 180.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	104.260 - 2.100
R-factor	0.187
Rwork	0.184
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2chi
RMSD bond length	0.018
RMSD bond angle	1.426
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.240	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.070	0.480
Number of reflections	15349
<I/σ(I)>	33	7.8
Completeness [%]	99.7	100
Redundancy	20.9	21.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5		HANGING DROP. RESERVOIR: MPD 5% CACL2 4.7 MM, TRIS 50 MM PH 8.5. DROP: 1 UL PROTEIN (22 MG/ML) AND 1 UL RESERVOIR