2CLK

Tryptophan Synthase in complex with D-glyceraldehyde 3-phosphate (G3P)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	182.045, 59.558, 67.409
Unit cell angles	90.00, 94.56, 90.00

Refinement procedure

Resolution	20.000 - 1.500
R-factor	0.18
Rwork	0.179
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	2.101
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.600
High resolution limit [Å]	1.500	1.500
Rmerge	0.060	0.230
Number of reflections	107933
<I/σ(I)>	8.3	1.9
Completeness [%]	97.3	83.1
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.8		pH 7.80