2CLA
CRYSTAL STRUCTURE OF THE ASP-199-ASN MUTANT OF CHLORAMPHENICOL ACETYLTRANSFERASE TO 2.35 ANGSTROMS RESOLUTION. STRUCTURAL CONSEQUENCES OF DISRUPTION OF A BURIED SALT-BRIDGE
Experimental procedure
| Spacegroup name | H 3 2 |
| Unit cell lengths | 107.700, 107.700, 124.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.350 |
| R-factor | 0.152 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.040 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.350 * |
| Rmerge | 0.078 * |
| Completeness [%] | 75.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 6.3 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5 (mg/ml) | |
| 2 | 1 | 1 | MES | 10 (mM) | |
| 3 | 1 | 2 | MPD | 4 (%) | |
| 4 | 1 | 2 | MES | 10 (mM) | |
| 5 | 1 | 2 | Cm | 1 (mM) | |
| 6 | 1 | 2 | hexaamminecobalt(III)chloride | 0.5 (mM) | |
| 7 | 1 | 2 | beta-mercaptoethanol | 5 (mM) |
