2CKY
Structure of the Arabidopsis thaliana thiamine pyrophosphate riboswitch with its regulatory ligand
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.850, 98.670, 54.550 |
Unit cell angles | 90.00, 104.80, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.900 |
R-factor | 0.191 |
Rwork | 0.183 |
R-free | 0.25000 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 2.218 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.235 | 0.970 |
Number of reflections | 8918 | |
<I/σ(I)> | 7.6 | 1.6 |
Completeness [%] | 96.0 | 74 |
Redundancy | 3.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 10% 1,6-HEXANEDIOL 50MM SODIUM CACODYLATE PH7.0 10MM MGSO4 |