2CKX
Crystal structure of NgTRF1, double-stranded telomeric repeat binding factor from Nicotiana tabacum.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 290 |
Detector technology | CCD |
Collection date | 2006-01-26 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.122, 48.265, 52.006 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.2101 |
Rwork | 0.210 |
R-free | 0.23270 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.218 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.800 | 1.900 |
Rmerge | 0.040 | 0.060 |
Number of reflections | 9807 | |
Completeness [%] | 98.2 | 96.9 |
Redundancy | 2.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.1M TRIS PH 8.5, 25%(W/V) PEG3350 |