2CKO
Crystal structure of Human Choline Kinase alpha 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.180, 122.820, 132.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.150 |
| R-factor | 0.204 |
| Rwork | 0.199 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nw1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.371 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.110 | 0.620 |
| Number of reflections | 50560 | |
| <I/σ(I)> | 20.38 | 5.76 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 17.9 | 17.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.01 M MGCL2, 15% PEG 3350, 0.2M NAF, pH 7.50 |
