2CKL
Ring1b-Bmi1 E3 catalytic domain structure
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 120.046, 120.046, 27.096 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.21300 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.565 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.730 |
| Number of reflections | 15568 | |
| <I/σ(I)> | 5.9 | 1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.3 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10% PEG3350, 0.1M NAI, 0.05M BIS-TRIS-PROPANE PH7.5, pH 7.50 |
