2CJL
CRYSTAL STRUCTURE AND ENZYMATIC PROPERTIES OF A BACTERIAL FAMILY 19 CHITINASE REVEAL DIFFERENCES WITH PLANT ENZYMES
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.671, 74.378, 64.179 |
| Unit cell angles | 90.00, 108.57, 90.00 |
Refinement procedure
| Resolution | 74.330 - 1.500 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wvv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.158 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.330 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.076 | 0.480 |
| Number of reflections | 64934 | |
| <I/σ(I)> | 13.2 | 3 |
| Completeness [%] | 98.5 | 97.4 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
