2CJH

Lysine aminotransferase from M. tuberculosis in the internal aldimine form with bound substrate 2-ketoglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	103.347, 103.347, 98.305
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	89.440 - 2.000
R-factor	0.158
Rwork	0.156
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.593
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.530	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.100	0.480
Number of reflections	38141
<I/σ(I)>	11.9	2.9
Completeness [%]	93.0	96.5
Redundancy	4.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1