2CJ3
CRYSTAL STRUCTURE OF PLASTOCYANIN FROM A CYANOBACTERIUM, ANABAENA VARIABILIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | PHILIPS |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1988-12-15 |
| Detector | NRAF-NONIUS CAD4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.290, 68.480, 42.260 |
| Unit cell angles | 90.00, 109.97, 90.00 |
Refinement procedure
| Resolution | 39.840 - 1.700 |
| R-factor | 0.141 |
| Rwork | 0.140 |
| R-free | 0.16300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1plc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.434 |
| Data reduction software | WEIS |
| Data scaling software | PROTEIN |
| Phasing software | MERLOT |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 21041 | |
| Completeness [%] | 80.1 | 24.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 288 | PEG6000, HEPES, POTASSIUM PHOSPHATE, SODIUM PHOSPHATE, PH 7.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 288K |
