2CIU
Structure of the IMS domain of the mitochondrial import protein Tim21 from S. cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-29 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.310, 58.820, 62.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.1975 |
Rwork | 0.198 |
R-free | 0.24830 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.680 |
High resolution limit [Å] | 1.600 | 1.580 |
Rmerge | 0.060 | 0.330 |
Number of reflections | 16515 | |
<I/σ(I)> | 18 | 3.72 |
Completeness [%] | 97.5 | 94 |
Redundancy | 3.33 | 3.27 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 0.2 M LI2SO4, 0.1 M BIS-TRIS PH 6.5, 25% PEG 3350 |