2CII
The crystal structure of H-2Db complexed with a partial peptide epitope suggests an MHC Class I assembly-intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.996, 58.450, 73.534 |
| Unit cell angles | 90.00, 106.77, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.550 |
| R-factor | 0.2391 |
| Rwork | 0.239 |
| R-free | 0.29690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ce6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.331 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.610 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.050 | 0.160 |
| Number of reflections | 15880 | |
| <I/σ(I)> | 19.8 | 5.3 |
| Completeness [%] | 95.8 | 96.9 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 25% PEG 6000, 100MM AMMONIUM SULFATE, 100 MM SODIUM CHLORIDE, 50 MM MES PH 5.0 |
