2CIH
Recombinant human H ferritin, K86Q and E27D mutant, soaked with Zn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-22 |
| Detector | MARRESEARCH |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.823, 180.823, 180.823 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.260 - 1.500 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2chi |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.307 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.500 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 40938 | |
| <I/σ(I)> | 23 | 6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.1 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | HANGING DROP. RESERVOIR: MPD 5% CACL2 4.7 MM, HEPES 50 MM PH 7.5 DROP: 1 UL PROTEIN (27MG/ML) AND 1 UL RESERVOIR |
