2CI8
sh2 domain of human nck1 adaptor protein - uncomplexed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-17 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 62.854, 82.484, 44.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.260 - 1.800 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jyu |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.813 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 11119 | |
| <I/σ(I)> | 34.3 | 3.2 |
| Completeness [%] | 99.1 | 95.5 |
| Redundancy | 6.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | METHOD: HANGING-DROP, VAPOR-DIFFUSION AT 4 DEGREES C, PROTEIN: 8 MG/ML PROTEIN IN 20 MM TRIS/HCL, PH 8.0, RESERVOIR: 25% PEG5000 MME, 0.1M MES PH 6.5, 0.2M (NH4)2SO4, 0.1M GUANIDINE HCL. CRYOPROTECTANT: 20% MPD |
