2CI7
Crystal structure of Dimethylarginine Dimethylaminohydrolase I in complex with Zinc, high pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.380, 74.630, 81.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.480 - 1.600 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h70 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | 0.450 |
| Number of reflections | 35849 | |
| <I/σ(I)> | 10.4 | 4.5 |
| Completeness [%] | 98.4 | 96.7 |
| Redundancy | 7.1 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.05 M GLYCINE/HCL, 28% PEG 3350, PH 9.0 |
