2CI1
Crystal Structure of dimethylarginine dimethylaminohydrolase I in complex with S-nitroso-Lhomocysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.664, 79.910, 44.894 |
| Unit cell angles | 90.00, 108.58, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.080 |
| R-free | 0.14130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h70 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.120 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 114012 | |
| <I/σ(I)> | 15.7 | 4.1 |
| Completeness [%] | 98.6 | 96 |
| Redundancy | 3.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 100 MM CITRIC ACID/NAOH, 20-40% PEG 8000, 2 MM TCEP, PH 5.0 |
