2CHU
CeuE in complex with mecam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Collection date | 2005-11-05 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.072, 42.445, 57.523 |
| Unit cell angles | 91.29, 97.06, 107.34 |
Refinement procedure
| Resolution | 69.170 - 2.400 |
| R-factor | 0.174 |
| Rwork | 0.170 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.693 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.120 | 2.530 |
| High resolution limit [Å] | 2.000 | 2.400 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 23425 | |
| <I/σ(I)> | 13.2 | 3.7 |
| Completeness [%] | 91.7 | 91.2 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% PEG 3350, 2.5 MM ZINC ACETATE, MECAM, 10 MM TRIS, 75 MM NACL, pH 7.50 |
