2CHO
Bacteroides thetaiotaomicron hexosaminidase with O-GlcNAcase activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.685, 93.454, 98.828 |
| Unit cell angles | 75.51, 94.15, 77.15 |
Refinement procedure
| Resolution | 37.740 - 1.850 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.22000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.433 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.090 | 0.490 |
| Number of reflections | 135175 | |
| <I/σ(I)> | 13 | 3 |
| Completeness [%] | 97.0 | 96 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.5M NA ACETATE, 15% PEG 3500, 0.1M MES, PH6.0 20% GLYCEROL, pH 6.00 |
