2CHE

STRUCTURE OF THE MG2+-BOUND FORM OF CHEY AND MECHANISM OF PHOSPHORYL TRANSFER IN BACTERIAL CHEMOTAXIS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	45.710, 46.980, 53.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 * - 1.800
R-factor	0.19
RMSD bond length	0.020
RMSD bond angle	0.041
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.800 *
Rmerge	0.069 *
Total number of observations	49659 *
Number of reflections	10659 *
Completeness [%]	95.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	CheY protein	1 (mM)
2	1	drop	sodium acetate	50 (mM)
3	1	drop	ammonium sulfate	1.9 (M)
4	1	drop	sodium acetate	50 (mM)
5	1	drop	PEG400	1-2 (%)
6	1	reservoir	ammonium sulfate-sodium acetate solution	1.9 (M)