2CHD
Crystal structure of the C2A domain of Rabphilin-3A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-17 |
| Detector | BRUKER SMART 6000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.870, 39.160, 88.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.410 - 1.920 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rsy |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.752 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.410 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.050 | 0.280 |
| Number of reflections | 10435 | |
| <I/σ(I)> | 19 | 3 |
| Completeness [%] | 97.8 | 92.6 |
| Redundancy | 6.13 | 2.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG4000, 0.1M AMMONIUM SULPHATE, 0.1M SODIUM ACETATE, PH=4.6 |
