2CGP
CATABOLITE GENE ACTIVATOR PROTEIN/DNA COMPLEX, ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 115 |
| Detector technology | CCD |
| Collection date | 1996-02 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.190, 79.190, 140.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.237 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cgp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.063 * | 0.411 * |
| Number of reflections | 22805 | |
| <I/σ(I)> | 14 | 2.6 |
| Completeness [%] | 86.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | COMPLEX WAS CRYSTALLIZED AT PH 6.0 FROM 4-6% PEG, 20% ETHYLENE GLYCOL, 0.2 M NACL, 25 MM MGCL2 AND 2 MM AMP. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | DNA | 1.3 molar excess | |
| 3 | 1 | reservoir | PEG3350 | 4-6 (%) | |
| 4 | 1 | reservoir | ethylene glycol | 20 (%) | |
| 5 | 1 | reservoir | 0.2 (M) | ||
| 6 | 1 | reservoir | 25 (mM) | ||
| 7 | 1 | reservoir | cAMP | 2 (mM) |
