2CGI

Siras structure of tetragonal lysozyme using derivative data collected at the high energy remote Holmium Kedge

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID15A
Synchrotron site	ESRF
Beamline	ID15A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-05-01
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	78.639, 78.639, 37.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.070 - 1.350
R-factor	0.176
Rwork	0.175
R-free	0.19600
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	1.143
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.400
High resolution limit [Å]	1.350	1.350
Rmerge	0.100
Number of reflections	26207
Completeness [%]	100.0	100
Redundancy	19.5	19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		pH 4.50