2CFO
Non-Discriminating Glutamyl-tRNA Synthetase from Thermosynechococcus elongatus in Complex with Glu
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-20 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.149, 99.603, 182.407 |
Unit cell angles | 90.00, 91.71, 90.00 |
Refinement procedure
Resolution | 45.600 - 2.450 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gln |
RMSD bond length | 0.017 |
RMSD bond angle | 1.540 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.110 | 0.400 |
Number of reflections | 46164 | |
<I/σ(I)> | 11.2 | 3.4 |
Completeness [%] | 96.9 | 91.8 |
Redundancy | 2.8 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.8 | 740 MM NA CITRATE, 140 MM CITRIC ACID, 10 MM DTT, pH 5.80 |