2CFO
Non-Discriminating Glutamyl-tRNA Synthetase from Thermosynechococcus elongatus in Complex with Glu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.149, 99.603, 182.407 |
| Unit cell angles | 90.00, 91.71, 90.00 |
Refinement procedure
| Resolution | 45.600 - 2.450 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gln |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.540 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.110 | 0.400 |
| Number of reflections | 46164 | |
| <I/σ(I)> | 11.2 | 3.4 |
| Completeness [%] | 96.9 | 91.8 |
| Redundancy | 2.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 740 MM NA CITRATE, 140 MM CITRIC ACID, 10 MM DTT, pH 5.80 |
