2CFE
The 1.5 A crystal structure of the Malassezia sympodialis Mala s 6 allergen, a member of the cyclophilin pan-allergen family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-24 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 71.989, 71.989, 106.178 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.740 - 1.500 |
R-factor | 0.143 |
Rwork | 0.143 |
R-free | 0.14900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cpl |
RMSD bond length | 0.015 |
RMSD bond angle | 1.629 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.700 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.060 | 0.310 |
Number of reflections | 45175 | |
<I/σ(I)> | 26.9 | 8.5 |
Completeness [%] | 99.0 | 100 |
Redundancy | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 1.5 M AMMONIUM SULFATE, 12% GLYCERIN, 0.1 M TRIS PH 8.5 |