2CF7

Asp74Ala mutant crystal structure for Dps-like peroxide resistance protein Dpr from Streptococcus suis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-19
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	106.930, 133.670, 135.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.280 - 1.500
R-factor	0.154
Rwork	0.153
R-free	0.17700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1umn
RMSD bond length	0.014
RMSD bond angle	1.486
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.590
High resolution limit [Å]	1.500	1.500
Rmerge	0.060	0.310
Number of reflections	308623
<I/σ(I)>	21	6
Completeness [%]	100.0	100
Redundancy	8	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	2UL AND 2UL VOLUME DROP, 30 % PEG 400, 0.2 M CACL2, 0.1 M HEPES-NAOH, PH 7.4, HANGING DROP, 16C