2CF4

Pyrococcus horikoshii TET1 peptidase can assemble into a tetrahedron or a large octahedral shell

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	F 41 3 2
Unit cell lengths	221.880, 221.880, 221.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.910 - 3.080
R-factor	0.256
Rwork	0.256
R-free	0.33200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vhe
RMSD bond length	0.014
RMSD bond angle	1.600
Data reduction software	XDS
Data scaling software	SELDAT
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.910	3.250
High resolution limit [Å]	3.080	3.080
Rmerge	0.070	0.220
Number of reflections	8651
<I/σ(I)>	31	5
Completeness [%]	95.2	75.8
Redundancy	22	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1