2CE4
Manganese Superoxide Dismutase (Mn-SOD) from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.280, 83.210, 59.520 |
| Unit cell angles | 90.00, 110.18, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.171 |
| Rwork | 0.168 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vew |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.308 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.160 |
| Number of reflections | 20567 | |
| <I/σ(I)> | 15.6 | 6 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 10 MM TRIS-HCL PH 8.5, 20MM MNCL2, 30% PEG 4000 |
