2CE1
Structure of reduced Arabidopsis thaliana cytochrome 6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 57.975, 57.975, 65.005 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.190 - 1.400 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.344 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.190 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.390 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 23823 | |
| <I/σ(I)> | 24 | 3 |
| Completeness [%] | 93.8 | 86 |
| Redundancy | 5.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 2.0 M AMMONIUM PHOSPHATE, 0.1 M TRIS HCL PH 8.5 |
