2CE0
Structure of oxidized Arabidopsis thaliana cytochrome 6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 58.209, 58.209, 64.761 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.990 - 1.240 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.277 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 40175 | |
| <I/σ(I)> | 24.8 | 4.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.1 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.26 M AMMONIUM SULFATE, 100 MM ACETATE PH 4.5 AND 200 MM NACL |
