2CDU
The Crystal Structure of Water-forming NAD(P)H Oxidase from Lactobacillus sanfranciscensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.636, 92.644, 163.465 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.650 - 1.800 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f8w |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.542 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.490 |
| Number of reflections | 81507 | |
| <I/σ(I)> | 29 | 1.9 |
| Completeness [%] | 96.1 | 73.8 |
| Redundancy | 8.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10 MG/ML PROTEIN, 100MM HEPES PH 7.0, 18% W/V PEG 4000, 8% V/V 2-PROPANOL, 20 MM DITHIOTHREITOL |
