2CDB
Sulfolobus solfataricus Glucose Dehydrogenase 1 in complex with NADP and glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.672, 91.394, 138.433 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 138.680 - 1.600 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cd9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.305 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.240 |
| Number of reflections | 203195 | |
| <I/σ(I)> | 13.9 | 4.1 |
| Completeness [%] | 96.5 | 95.6 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 % PEG 8000, 0.1 M TRIS (PH 8.0), 4.5 % (V/V) PROPAN-2-OL |
