2CD9
Sulfolobus solfataricus Glucose Dehydrogenase 1 - apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Collection date | 2004-06-12 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 68.501, 90.310, 138.941 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 141.420 - 1.800 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | THERMOPLASMA ACIDOPHILUM GDH |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.509 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.100 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.270 |
| Number of reflections | 73910 | |
| <I/σ(I)> | 12.8 | 4 |
| Completeness [%] | 96.7 | 92.9 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12 % PEG, 0.1 M MES (PH 5.75), 3% (V/V) PROPAN-2-OL |
