2CD9

Sulfolobus solfataricus Glucose Dehydrogenase 1 - apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Collection date	2004-06-12
Spacegroup name	P 21 21 2
Unit cell lengths	68.501, 90.310, 138.941
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	141.420 - 1.800
R-factor	0.193
Rwork	0.191
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	THERMOPLASMA ACIDOPHILUM GDH
RMSD bond length	0.016
RMSD bond angle	1.509
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.100	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.070	0.270
Number of reflections	73910
<I/σ(I)>	12.8	4
Completeness [%]	96.7	92.9
Redundancy	3.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				12 % PEG, 0.1 M MES (PH 5.75), 3% (V/V) PROPAN-2-OL