2CD0
STRUCTURE OF HUMAN LAMBDA-6 LIGHT CHAIN DIMER WIL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-09 |
| Detector | CUSTOM-MADE |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 147.900, 147.900, 46.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| Rwork | 0.289 |
| R-free | 0.35100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MCG (1DCL): USED ONLY VL DIMER WITH CDR LOOPS DELETED. |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.232 |
| Number of reflections | 21478 | |
| <I/σ(I)> | 48 | 19 |
| Completeness [%] | 97.6 | 96.4 |
| Redundancy | 21.8 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 1.8M AMMONIUM SULPHATE, 0.1 M SODIUM CITRATE PH 5.6, 0.01 M CADMIUM CHLORIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | ||
| 2 | 1 | reservoir | PEG6000 |
