2CBR
CELLULAR RETINOIC ACID BINDING PROTEIN I IN COMPLEX WITH A RETINOBENZOIC ACID (AM80)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 133.500, 133.500, 40.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| Rwork | 0.230 |
| R-free | 0.26600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cbr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 * | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.086 | 0.260 |
| Number of reflections | 10128 | |
| <I/σ(I)> | 20.2 | 5.2 |
| Completeness [%] | 97.6 | 95.7 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 277 * | drop contained equal volume of protein and reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 19 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | beta-octyl glucoside | 0.1 (%) | |
| 4 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 30 (%) | |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) |
