2CBC

STRUCTURE OF NATIVE AND APO CARBONIC ANHYDRASE II AND SOME OF ITS ANION-LIGAND COMPLEXES

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	42.700, 41.700, 73.000
Unit cell angles	90.00, 104.60, 90.00

Refinement procedure

Resolution	10.000 - 1.880
R-factor	0.166
RMSD bond length	0.021
RMSD bond angle	0.036
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	3.410 *
High resolution limit [Å]	1.880 *	1.880 *
Rmerge	0.056 *	0.180 *
Total number of observations	54880 *
Number of reflections	18395 *	1613 *
Completeness [%]	89.3 *	89.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	2.4 (M)
2	1	1	Tris-HCl	50 (mM)
3	1	1		1 (mM)