2CAM
AVIDIN MUTANT (K3E,K9E,R26D,R124L)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.590, 82.150, 43.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.200 - 2.200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ave |
| RMSD bond length | 0.015 |
| RMSD bond angle | 20.493 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.700 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.045 | |
| Total number of observations | 49395 * | |
| Number of reflections | 13827 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 95.0 | 93.2 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.2 * | 22 * | RECOMBINANT AVIDIN WAS CRYSTALLIZED FROM AMMONIUM SULFATE 2M. PH 5.7 0.05 M PHOSPHATE BUFFER AT 22 C BY VAPOUR DIFFUSION TECHNIQUES., vapor diffusion, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 3 | 1 | reservoir | sodium phosphate | 0.05 (M) | pH5.7 |
| 4 | 1 | drop | PEG8000 | 9 (%mass/v) | |
| 5 | 1 | drop | Tris-HCl | 0.05 (M) | pH7.2 |
