2CAM

AVIDIN MUTANT (K3E,K9E,R26D,R124L)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-12
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 2
Unit cell lengths	76.590, 82.150, 43.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.200 - 2.200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ave
RMSD bond length	0.015
RMSD bond angle	20.493 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.000	2.700
High resolution limit [Å]	2.200	2.200
Rmerge	0.045
Total number of observations	49395 *
Number of reflections	13827
<I/σ(I)>	15
Completeness [%]	95.0	93.2
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.2 *	22 *	RECOMBINANT AVIDIN WAS CRYSTALLIZED FROM AMMONIUM SULFATE 2M. PH 5.7 0.05 M PHOSPHATE BUFFER AT 22 C BY VAPOUR DIFFUSION TECHNIQUES., vapor diffusion, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	2.0 (M)
3	1	reservoir	sodium phosphate	0.05 (M)	pH5.7
4	1	drop	PEG8000	9 (%mass/v)
5	1	drop	Tris-HCl	0.05 (M)	pH7.2