2CAK
1.27Angstrom Structure of Rusticyanin from Thiobacillus ferrooxidans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.473, 60.823, 38.121 |
| Unit cell angles | 90.00, 108.01, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.270 |
| R-factor | 0.1207 |
| R-free | 0.13780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a8z |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 1.300 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.050 | 0.390 |
| Number of reflections | 37281 | |
| <I/σ(I)> | 9.7 | 2.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.8 | pH 3.80 |
