2CAI
Structure of Glutathione-S-Transferase mutant, R21L, from Schistosoma Haematobium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-14 |
Detector | MARRESEARCH |
Spacegroup name | I 2 3 |
Unit cell lengths | 148.716, 148.716, 148.716 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 105.410 - 2.260 |
R-factor | 0.218 |
Rwork | 0.215 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oe7 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.508 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.300 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.060 | |
Number of reflections | 25780 | |
<I/σ(I)> | 10.2 | 9.8 |
Completeness [%] | 99.1 | 99.8 |
Redundancy | 22 | 20.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 10% PEG200, PBS PH7.4, 5MM MERCAPTOETHANOL, pH 7.40 |