2CAI
Structure of Glutathione-S-Transferase mutant, R21L, from Schistosoma Haematobium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-14 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 148.716, 148.716, 148.716 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 105.410 - 2.260 |
| R-factor | 0.218 |
| Rwork | 0.215 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oe7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.508 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.060 | |
| Number of reflections | 25780 | |
| <I/σ(I)> | 10.2 | 9.8 |
| Completeness [%] | 99.1 | 99.8 |
| Redundancy | 22 | 20.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 10% PEG200, PBS PH7.4, 5MM MERCAPTOETHANOL, pH 7.40 |
