2CA1
Crystal structure of the IBV coronavirus nucleocapsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 43 |
| Unit cell lengths | 61.590, 61.590, 91.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.2031 |
| Rwork | 0.203 |
| R-free | 0.23560 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.352 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.700 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 | 0.420 |
| Number of reflections | 31078 | |
| <I/σ(I)> | 16.23 | 3.1 |
| Completeness [%] | 90.6 | 90.5 |
| Redundancy | 1.65 | 1.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.50 |
