2C9U
1.24 Angstroms resolution structure of as-isolated Cu-Zn Human Superoxide dismutase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-10 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.638, 67.553, 52.320 |
Unit cell angles | 90.00, 106.48, 90.00 |
Refinement procedure
Resolution | 50.190 - 1.240 |
R-factor | 0.141 |
Rwork | 0.139 |
R-free | 0.17600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hl5 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.841 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.280 |
High resolution limit [Å] | 1.240 | 1.240 |
Rmerge | 0.060 | 0.400 |
Number of reflections | 70045 | |
<I/σ(I)> | 20 | 2.2 |
Completeness [%] | 96.3 | 92.5 |
Redundancy | 3.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 3.0 M AMMONIUM SULPHATE, 100 MM SODIUM CHLORITE, 50MM SODIUM ACETATE BUFFER, pH 8.00 |