2C9R
apo-H91F CopC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | I 41 |
Unit cell lengths | 72.315, 72.315, 47.542 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.740 - 2.000 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c9q |
RMSD bond length | 0.010 |
RMSD bond angle | 1.384 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.040 | 0.240 |
Number of reflections | 8189 | |
<I/σ(I)> | 23.8 | 3.7 |
Completeness [%] | 97.7 | 95.6 |
Redundancy | 2.4 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.6 | 2.4 M SODIUM ACETATE, PH 7.0, 0.1 M BIS-TRIS PROPANE, PH 6.6, EDTA, 10 MM. |