2C9Q

Cu(I)Cu(II)-CopC at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	55.781, 55.781, 60.261
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.600
R-factor	0.189
Rwork	0.187
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c9p
RMSD bond length	0.012
RMSD bond angle	1.539
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.070	0.320
Number of reflections	13027
<I/σ(I)>	33	7.9
Completeness [%]	99.6	100
Redundancy	13.2	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		2.0 M AMMONIUM SULFATE, 0.1 M SODIUM HEPES, PH 7.5, 2% (W/V) PEG 400