2C9M
Structure of (SR) Calcium-ATPase in the Ca2E1 state solved in a P1 crystal form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-13 |
| Detector | MARRESEACH |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.948, 81.276, 131.006 |
| Unit cell angles | 97.64, 99.94, 95.22 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.266 |
| Rwork | 0.266 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1su4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.060 | 0.340 |
| Number of reflections | 41479 | |
| <I/σ(I)> | 12.1 | 2.3 |
| Completeness [%] | 80.0 | 40 |
| Redundancy | 1.9 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 14% PEG 6000, 400 MM CACL2, pH 6.80 |
