2C9I
Structure of the fluorescent protein asFP499 from Anemonia sulcata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.880, 135.126, 95.071 |
| Unit cell angles | 90.00, 106.93, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.820 |
| R-factor | 0.248 |
| Rwork | 0.245 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.387 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.050 | 0.190 |
| Number of reflections | 128068 | |
| <I/σ(I)> | 17.5 | 2 |
| Completeness [%] | 87.3 | 81.4 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 30 PERCENT PEG 4000, 0.1 M TRIS PH 8.5, 0.2 M MAGNESIUM CHLORIDE |
